NanoPlot report

Summary statistics

General summary: barcode11 barcode12 unclassified barcode01 barcode08 barcode04 barcode02 barcode07 barcode09 barcode03 barcode10 barcode06 barcode05

Active channels: 242.0 230.0 218.0 16.0 16.0 17.0 29.0 19.0 16.0 18.0 12.0 17.0 16.0

Mean read length: 1,647.2 1,452.2 1,483.2 1,018.1 1,374.5 1,353.2 1,744.4 1,503.0 1,471.5 2,047.6 1,615.7 964.3 1,486.2

Mean read quality: 8.0 8.0 5.3 5.0 5.3 5.1 5.1 5.4 5.2 5.6 5.0 5.0 5.0

Median read length: 1,219.0 1,008.5 993.0 793.5 1,106.0 863.5 1,098.0 1,307.5 1,324.5 932.0 1,181.0 703.0 1,058.0

Median read quality: 8.2 8.1 5.0 4.8 4.8 5.1 4.9 5.2 5.2 4.9 5.1 5.1 4.4

Number of reads: 12,783.0 5,692.0 2,685.0 18.0 17.0 20.0 33.0 22.0 20.0 22.0 13.0 19.0 16.0

Read length N50: 2,336.0 2,013.0 2,234.0 1,499.0 1,763.0 1,894.0 2,463.0 1,995.0 1,777.0 4,607.0 2,571.0 1,605.0 2,510.0

Total bases: 21,055,523.0 8,266,183.0 3,982,482.0 18,326.0 23,367.0 27,064.0 57,564.0 33,067.0 29,430.0 45,048.0 21,004.0 18,321.0 23,779.0

Number, percentage and megabases of reads above quality cutoffs

>Q5:

12405 (97.0%) 20.5Mb

5505 (96.7%) 8.0Mb

1321 (49.2%) 1.9Mb

7 (38.9%) 0.0Mb

8 (47.1%) 0.0Mb

11 (55.0%) 0.0Mb

16 (48.5%) 0.0Mb

13 (59.1%) 0.0Mb

12 (60.0%) 0.0Mb

10 (45.5%) 0.0Mb

7 (53.8%) 0.0Mb

10 (52.6%) 0.0Mb

7 (43.8%) 0.0Mb

>Q7:

9748 (76.3%) 16.6Mb

4232 (74.3%) 6.4Mb

383 (14.3%) 0.6Mb

1 (5.6%) 0.0Mb

2 (11.8%) 0.0Mb

2 (10.0%) 0.0Mb

3 (9.1%) 0.0Mb

2 (9.1%) 0.0Mb

1 (5.0%) 0.0Mb

5 (22.7%) 0.0Mb

1 (7.7%) 0.0Mb

0 (0.0%) 0.0Mb

2 (12.5%) 0.0Mb

>Q10:

1065 (8.3%) 2.0Mb

429 (7.5%) 0.8Mb

16 (0.6%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

>Q12:

11 (0.1%) 0.0Mb

1 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

>Q15:

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

0 (0.0%) 0.0Mb

Top 5 highest mean basecall quality scores and their read lengths

1:

13.1 (5045; 01f26b18-75ed-4e72-87ee-a74f1202dc94)

12.1 (533; 03240cbf-1665-49cd-93e5-6c5559fe688a)

11.9 (1248; 03ac446c-1e62-4c8d-b4ed-8cc96219a98c)

8.0 (498; 076fe8a1-41cf-4758-b6d7-5099d8e38351)

9.0 (1456; 20f74024-8e0b-42e3-a1b1-260ede1abfc4)

8.0 (295; 5dad8c7b-4a6b-48c1-a28c-cbd7af0c4afe)

8.2 (1035; 3b4f0c88-4602-4b69-a2da-6b4d18437435)

7.8 (2783; 864754ad-dde9-4d57-b899-233738fcdc73)

7.2 (2224; e1de5db6-a9b4-426e-94be-9e6a7fce672b)

9.7 (861; 32dbe3aa-f70b-41d0-bc1f-4fff8b53e198)

7.8 (2673; 0b69c685-9d33-4a34-8c1c-f2314476ee0a)

6.4 (375; 4470b2b1-b40a-47c0-a044-5013bea78598)

8.2 (289; 8517c500-3edb-4d37-a05f-ccb9f5626819)

2:

12.6 (2895; 87e337b5-01f3-40b1-ac0f-43b9c711c676)

11.9 (509; a1de3705-9477-4e8c-8f7d-bcd602360da8)

11.3 (1582; ea99ec73-4abb-44ca-b375-a8e3d9d7a386)

6.8 (565; d718561f-82f9-40c4-93d7-96456a7d7fc9)

7.4 (1882; 909da83e-e1d2-4c75-8380-0ebe77a13828)

7.5 (664; 25505a1c-66c2-49cf-83cd-ffd6c415226a)

7.5 (2014; 49219118-97d7-470e-ad99-bade7ddc1b46)

7.2 (1424; 35bb9b46-1abc-4b4a-ac23-3fd740d9134f)

6.9 (768; 59a9b86a-0cc5-4c3d-8050-e37dcd174c41)

8.1 (820; 95ef6fb9-76ae-4518-a8e8-bb421c9c84dd)

6.0 (544; 59edb11b-188d-42d4-8710-0951b3e78917)

6.1 (1605; 16f38715-0456-497d-840f-a3ba58e86717)

8.1 (1717; 6b230700-3347-42a9-9464-2a454bb5d4fe)

3:

12.5 (4040; 54376d93-fdde-4ebf-a26d-ba7c2dcf4f6d)

11.8 (1078; 84cb3d3a-7a7c-4b1a-999e-6d120e29a598)

10.7 (1167; 57716b29-db4a-4f2c-9d03-0a6d9ea4e30f)

6.6 (834; 4fa0444a-7be6-437b-b64b-75498079dfdf)

6.6 (192; cfb939b0-8447-4edf-8601-f885ad6cbfca)

7.0 (1123; 8365ad7a-e41f-4a9f-8dea-d1fd69641d90)

7.2 (2074; e78d6eb7-d55f-4581-8273-a7eea1e3a2dd)

6.7 (5055; c483166e-7870-4a83-b7b3-8bac480b160a)

6.8 (3566; 863052f5-d97d-443b-96e9-2010273077a7)

7.8 (4622; b32fc428-3de1-4431-88dd-b9123ace3ab8)

5.4 (1741; 685e5ef9-b6be-41bf-8856-626a380cf3e1)

6.1 (162; 0505983c-0eb7-4b62-93a4-18c441bb57fc)

5.9 (226; 9efdb2f1-5ae4-4462-b562-8b8786207f23)

4:

12.4 (1606; 60498683-5238-4d16-945d-c90fa1be4bac)

11.8 (2407; e1c64a9e-76da-4892-a676-887563ddbeae)

10.7 (1473; f5df7e73-9213-4a27-b400-40fd682717b9)

5.7 (753; a5c22465-ac20-4c9d-a548-1f89c2f91740)

6.1 (867; e8337d5f-cf8e-424c-a50f-2688ac35b158)

6.5 (749; 52932792-9b8f-4291-b8e5-67e71515ab63)

6.8 (4441; 25e94182-e545-4501-8c98-d4ae585f6614)

6.4 (1491; c031d64d-412d-4557-95ac-fad0d7174fe0)

6.4 (413; 2707134f-3e7b-4022-b915-694cc1a186d3)

7.7 (458; 4e72c4a4-b606-45b2-afef-4c3b1bd88588)

5.4 (926; 777e6d74-f702-483c-be61-382e37e36e0c)

5.9 (2322; 5f08cb3c-fe64-436f-97e9-5a7993aa46f3)

5.6 (3573; b69034de-ce78-4a47-b2b1-10c0d035525e)

5:

12.3 (574; a046a983-e980-4aec-a59f-572516595d44)

11.8 (1087; 402e12d5-4533-4a68-bd90-1f0c293b35ac)

10.5 (599; 2e59f771-eb0b-4547-be58-6448028e72ab)

5.6 (3186; 56e65600-ab88-4af3-8dfc-9855bf3d56e0)

6.0 (2283; 90065f4b-d6e3-4bd0-ba6e-232658a9196e)

6.2 (4989; b6889440-b05e-44b7-8d86-fed617786fdd)

6.6 (393; 16a0dbab-3938-4f04-927d-61a8df2e6c0a)

6.3 (863; 13d6950d-0010-4c7d-b6a6-2d133800338c)

6.0 (1777; 14b4c0d7-68a2-4e99-9d03-a1498473436d)

7.3 (790; 0deb9d54-85cb-40e4-8dc8-112271cfe6ec)

5.3 (142; 0c2a4abc-ceac-4ce8-b844-f2cfb881518d)

5.3 (524; 14583dfa-c4be-4026-8560-9b736732450d)

5.5 (1870; 8bec517c-b8cc-4951-a39f-2cfba6871d5c)

Top 5 longest reads and their mean basecall quality score

1:

8836 (9.6; 2c1ebcc4-f6f1-4bd0-8c44-4522e525c705)

10185 (6.5; 072b589b-fe23-4647-bcd7-fb860a7f748b)

22666 (3.4; a186acb5-ba17-41c8-828e-49d12b8d7e7f)

3186 (5.6; 56e65600-ab88-4af3-8dfc-9855bf3d56e0)

2908 (4.2; 02f74ad2-4dba-4298-a48b-20554496a19e)

4989 (6.2; b6889440-b05e-44b7-8d86-fed617786fdd)

7529 (4.5; 779954fc-4d70-4737-8722-a01c8e64aecc)

5055 (6.7; c483166e-7870-4a83-b7b3-8bac480b160a)

3566 (6.8; 863052f5-d97d-443b-96e9-2010273077a7)

6326 (4.3; f43e3d7d-3c39-4890-8c4e-16f2d8f8279b)

6306 (4.8; 3b1dd028-bdfd-4057-bb1f-17517ab42c85)

2912 (5.1; d6620c02-09c3-49fe-baab-9b249f38bfac)

3585 (4.2; 83b2902a-1b62-4883-aebb-894f03c39726)

2:

8425 (8.9; fc36bb20-0790-42c3-8f15-154cd39bce92)

9276 (9.7; 2afdf98e-a971-41a0-ba4b-517958e036a6)

14611 (3.5; 655e07ec-45ac-4ac6-a89f-ff1efbb17d58)

1830 (4.0; 7aa5e173-9a5f-47f2-865b-33f68a8b415d)

2479 (4.6; cab94e39-6bd6-4f29-8d7e-06443e203b40)

2777 (3.6; ee69d89e-5a62-4305-807b-681cc7b5490a)

6561 (3.4; ce768b00-eb7e-48e4-a631-1b18febd82fe)

3184 (5.8; e90dcc69-c66f-4228-9adc-ab7d64434fff)

2899 (4.9; 6b71b8a0-4242-482a-a8d6-b6a866a06c93)

5213 (4.0; bb393097-dced-4868-8f64-90a71bdddcdc)

2673 (7.8; 0b69c685-9d33-4a34-8c1c-f2314476ee0a)

2661 (4.5; a1377821-fe80-4e6c-9706-f8950155f45d)

3573 (5.6; b69034de-ce78-4a47-b2b1-10c0d035525e)

3:

8247 (8.3; 32309ef0-eb74-4406-8958-570fbc8a723c)

9054 (10.0; a11d4753-db26-4bb7-8ee7-8643cc8f314b)

8700 (4.0; cda79166-42ae-4f7c-a4f4-096f5d12f61d)

1644 (5.3; 267d57c4-c439-4981-8cf2-ebae3d042159)

2283 (6.0; 90065f4b-d6e3-4bd0-ba6e-232658a9196e)

2496 (4.0; 2f4c5996-e027-496b-9bfb-8b44170ac4bd)

4441 (6.8; 25e94182-e545-4501-8c98-d4ae585f6614)

2783 (7.8; 864754ad-dde9-4d57-b899-233738fcdc73)

2639 (5.6; 34d5bbb3-9565-42df-9b66-97dbf77c464f)

4793 (4.3; 05196ce7-d69c-4c3d-b4dd-032908f5b60a)

2571 (4.0; 88a53483-64b9-4b30-bc20-730631468c38)

2322 (5.9; 5f08cb3c-fe64-436f-97e9-5a7993aa46f3)

2573 (3.7; 1db6311f-d640-47d7-8406-35fc46b7b9df)

4:

8232 (11.1; 0e2e9026-f2af-4d68-a01a-4b6c52b026a8)

8800 (7.0; 9d5fbd13-eef6-419f-abc6-9e2f7c83c130)

8095 (7.3; be888e21-d241-40e9-a970-11bd6ef554da)

1506 (4.1; 20be683b-6ba5-49bc-b9c1-d0948a6a8009)

2031 (3.7; 0e602dd0-f7f1-49b3-acab-7d59d7a6920c)

2431 (5.2; b7dcbeef-a81b-4dd0-8026-5711992f2735)

4162 (3.9; d04fc439-8b54-4f4f-9956-14e41bf22485)

2579 (3.3; 4369f8c5-3a9c-42b4-b2d8-6f7a827bd1ce)

2348 (4.6; bbfc4fa3-0da6-46e6-add4-4db1a26907cb)

4622 (7.8; b32fc428-3de1-4431-88dd-b9123ace3ab8)

1959 (5.3; 676928cd-2fbe-4d34-aab1-ad986b25806b)

1605 (6.1; 16f38715-0456-497d-840f-a3ba58e86717)

2510 (3.9; 672a5572-1daa-4f26-9f5a-dfa203711872)

5:

8226 (9.2; d146e5ac-6575-4b52-a3b8-7375a27e6177)

8637 (6.7; 91c962d4-1ae3-4f5a-98c5-cde49a2f4a2a)

8002 (3.6; 2ecaf5d2-c384-4181-9fd8-420ec02f6868)

1499 (5.0; 557b19d6-e796-4c1d-a750-056a6074ff71)

1882 (7.4; 909da83e-e1d2-4c75-8380-0ebe77a13828)

1894 (5.9; 45237b0d-1b49-46bd-adc3-6e26c792a6e2)

3199 (6.2; 7b2dd78a-4ea1-4770-bb4d-9def37d42ad1)

2563 (5.0; 07206c34-65c5-4257-be4e-83f51fb04467)

2224 (7.2; e1de5db6-a9b4-426e-94be-9e6a7fce672b)

4607 (6.0; af4da727-adb5-4531-88bb-617e396569e6)

1741 (5.4; 685e5ef9-b6be-41bf-8856-626a380cf3e1)

1336 (5.2; cab1009c-277d-422f-9db1-06b533e12851)

2314 (4.2; 85633e37-6313-444c-8e94-fd464133309e)

Plots

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots after log transformation of read lengths

Read lengths vs Average read quality plot using a kernel density estimation after log transformation of read lengths

Number of reads generated per channel

Cumulative yield

Cumulative yield

Number of reads over time

Number of active pores over time

Violin plot of read lengths over time

Violin plot of quality over time

Violin plot of sequencing speed over time

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots after log transformation of read lengths

Read lengths vs Average read quality plot using a kernel density estimation after log transformation of read lengths

Number of reads generated per channel

Cumulative yield

Cumulative yield

Number of reads over time

Number of active pores over time

Violin plot of read lengths over time

Violin plot of quality over time

Violin plot of sequencing speed over time

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots after log transformation of read lengths

Read lengths vs Average read quality plot using a kernel density estimation after log transformation of read lengths

Number of reads generated per channel

Cumulative yield

Cumulative yield

Number of reads over time

Number of active pores over time

Violin plot of read lengths over time

Violin plot of quality over time

Violin plot of sequencing speed over time

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots after log transformation of read lengths

Read lengths vs Average read quality plot using a kernel density estimation after log transformation of read lengths

Number of reads generated per channel

Cumulative yield

Cumulative yield

Number of reads over time

Number of active pores over time

Violin plot of read lengths over time

Violin plot of quality over time

Violin plot of sequencing speed over time

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots after log transformation of read lengths

Read lengths vs Average read quality plot using a kernel density estimation after log transformation of read lengths

Number of reads generated per channel

Cumulative yield

Cumulative yield

Number of reads over time

Number of active pores over time

Violin plot of read lengths over time

Violin plot of quality over time

Violin plot of sequencing speed over time

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots after log transformation of read lengths

Read lengths vs Average read quality plot using a kernel density estimation after log transformation of read lengths

Number of reads generated per channel

Cumulative yield

Cumulative yield

Number of reads over time

Number of active pores over time

Violin plot of read lengths over time

Violin plot of quality over time

Violin plot of sequencing speed over time

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots after log transformation of read lengths

Read lengths vs Average read quality plot using a kernel density estimation after log transformation of read lengths

Number of reads generated per channel

Cumulative yield

Cumulative yield

Number of reads over time

Number of active pores over time

Violin plot of read lengths over time

Violin plot of quality over time

Violin plot of sequencing speed over time

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots after log transformation of read lengths

Read lengths vs Average read quality plot using a kernel density estimation after log transformation of read lengths

Number of reads generated per channel

Cumulative yield

Cumulative yield

Number of reads over time

Number of active pores over time

Violin plot of read lengths over time

Violin plot of quality over time

Violin plot of sequencing speed over time

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots after log transformation of read lengths

Read lengths vs Average read quality plot using a kernel density estimation after log transformation of read lengths

Number of reads generated per channel

Cumulative yield

Cumulative yield

Number of reads over time

Number of active pores over time

Violin plot of read lengths over time

Violin plot of quality over time

Violin plot of sequencing speed over time

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots after log transformation of read lengths

Read lengths vs Average read quality plot using a kernel density estimation after log transformation of read lengths

Number of reads generated per channel

Cumulative yield

Cumulative yield

Number of reads over time

Number of active pores over time

Violin plot of read lengths over time

Violin plot of quality over time

Violin plot of sequencing speed over time

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots after log transformation of read lengths

Read lengths vs Average read quality plot using a kernel density estimation after log transformation of read lengths

Number of reads generated per channel

Cumulative yield

Cumulative yield

Number of reads over time

Number of active pores over time

Violin plot of read lengths over time

Violin plot of quality over time

Violin plot of sequencing speed over time

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots after log transformation of read lengths

Read lengths vs Average read quality plot using a kernel density estimation after log transformation of read lengths

Number of reads generated per channel

Cumulative yield

Cumulative yield

Number of reads over time

Number of active pores over time

Violin plot of read lengths over time

Violin plot of quality over time

Violin plot of sequencing speed over time

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots after log transformation of read lengths

Read lengths vs Average read quality plot using a kernel density estimation after log transformation of read lengths

Number of reads generated per channel

Cumulative yield

Cumulative yield

Number of reads over time

Number of active pores over time

Violin plot of read lengths over time

Violin plot of quality over time

Violin plot of sequencing speed over time